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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in lots of tumors, which leads to chromosome instability, and therefore may well add to tumorigenesis. This protein was identified to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical part in endorsing mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are described. Two extra splice variants were reported but comprehensive nucleotide sequence info is not really but out there.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the absolute best benefits are...
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This tutorial outlines more achievable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind on the epitope around the focus on protein. By evaluating the staining with the blocked antibody vs . the antibody alone, one can see which staining is certain.
Focus on Information and facts Cyclin E belongs for the remarkably conserved cyclin family, whose associates are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to your temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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